FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification
نویسندگان
چکیده
منابع مشابه
Absolute quantification of murine interleukine-4, interleukine- 10 and interferon-γ gene transcripts using Real Time PCR
The study of cytokines gene expression is quite important in various conditions of health and disease for the evaluation of clinical responses to new vaccination approaches. An absolute quantification is based on a calibration curve and production of standard controls to achieve more reliable results than in relative system. In this study we attempted to construct standard controls to evaluate ...
متن کاملdevelopment of quantitative competitive pcr and absolute based real-time pcr assays for quantification of the butyrate producing bacterium: butyrivibrio fibrisolvens
introduction butyrivibrio fibrisolvens strains are presently recognized as the major butyrate-producing bacteria found in the rumen and digestive track of many animals and also in the human gut. in this study we reported the development of two dna based techniques, quantitative competitive (qc) pcr and absolute based real-time pcr, for enumerating butyrivibrio fibrisolvens strains. despite the ...
متن کاملDesign a Real Time PCR with SYBR Green for quantification of HTLV-1 proviral load for blood donors
Abstract Background and Objectives In Iran, Khorasan province is an endemic area for HTLV-1 virus. Considering the inability of serological tests to determine HTLV-1 in window period, their failure to confirm the indetermination results of western blot, and given the probability for HTLV-1 transfusion transmission, a SYBR green-based Real Time PCR was set to measure the HTLV-1 proviral load. ...
متن کاملTissue-specific Calibration of Real-time PCR Facilitates Absolute Quantification of Plasmid DNA in Biodistribution Studies
Analysis of the tissue distribution of plasmid DNA after administration of nonviral gene delivery systems is best accomplished using quantitative real-time polymerase chain reaction (qPCR), although published strategies do not allow determination of the absolute mass of plasmid delivered to different tissues. Generally, data is expressed as the mass of plasmid relative to the mass of genomic DN...
متن کاملabsolute quantification of murine interleukine-4, interleukine- 10 and interferon-γ gene transcripts using real time pcr
the study of cytokines gene expression is quite important in various conditions of health and disease for the evaluation of clinical responses to new vaccination approaches. an absolute quantification is based on a calibration curve and production of standard controls to achieve more reliable results than in relative system. in this study we attempted to construct standard controls to evaluate ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2008
ISSN: 1471-2105
DOI: 10.1186/1471-2105-9-13